DNA barcoding – ABOL – The Austrian Barcode of Life

On the first weekend in July, the ABOL-BioBlitz was a guest at the GEO Day of Biodiversity in the Geopark Karawanken, this year for the first time in southern Carinthia. After the welcome and introductory words in Bleiburg, the Globasnitzbachtal was investigated in the afternoon and evening of the first day. There was something for everyone there – stream, forests, and also wonderful species-rich meadows that made botanists and entomologists’ hearts beat faster. On the second day, the high elevations of the Petzen were the target of the species survey. Equipped with cameras, nets, tubes, etc., the cable car took us to the study area. The sunny weather provided good conditions for the species search, only the wind in exposed locations was a bit of a hindrance. Due to the geological peculiarities and the southern influence, the region is particularly species-rich and therefore extremely exciting for all researchers. Initial estimates have already shown that around 1200 species have been found, which is an impressive number. The success is due not least to the large number of around 90 participating scientists, as well as the perfect organization of the event by the state of Carinthia, the Ökoteam and E.C.O.

We would like to thank you very much for allowing us to be there again, for the great food, the pretty t-shirts and the opportunity to meet friends and colleagues as well as new people.

We would like to call on collectors to contact us if they would like to provide samples of arthropods for DNA barcoding!

17. July, 2024/by Michaela Sonnleitner

N. Fial

10 years ÖEG insect camp

ABOL BioBlitz, Highlights, Veranstaltungen

Parallel to the Day of Biodiversity in the Biosphere Reserve Wienerwald, ABOL was represented for the third time at the ÖEG insect camp from June 6th to 9th. Like ABOL, the insect camp also celebrated its 10th anniversary this year, and this year it took place in the Karwendel Nature Reserve in Tyrol. On this occasion, all participants were surprised with lovingly designed T-shirts. With a smile and a tear in her eye, the ÖEG youth officer Elisabeth Huber ceremoniously handed over the organization of the camp to Elisabeth Glatzhofer and Samuel Messner.

Around 40 experts and aspiring young entomologists collected various arthropod groups on 13 sample areas. Butterflies, beetles, cicadas, hymenoptera, bugs, flies, millipedes and the like were well represented due to the good weather. The sample areas were as diverse as the taxa collected themselves. Among others, coniferous forests, alpine pastures, ponds, alpine limestone grasslands, high alpine scree slopes and the river bank along the Isar were examined.

In short we presented our new Biodiversity Fund project RefDat and invited all participants to join the project. All interested parties could then pick up tubes prepared by our team for sampling the collected material.

Early in the morning, with full stomachs, a wide range of utensils and mostly ideal weather, the groups fanned out to the study area. After dinner, they met in the restaurant and prepared and identified in a relaxed atmosphere until late into the night. During this time, the opportunity was taken to exchange profound information and make new contacts. All the brave and bright ones went out to light up the night thunderstorms to catch moths with light traps at the end of the day.

We would like to thank the ÖEG team and the Karwendel Nature Park team for the great catering and organization and especially Elisabeth Huber for her many years of commitment! We are eagerly awaiting the results of the data analysis as well as the results of our DNA samples.

Impressions (photos: N. Fial, N. Szucsich):

17. July, 2024/by Michaela Sonnleitner

TdA Biosphere Reserve Wienerwald - Vienna-Liesing

M. Sonnleitner

The first ABOL BioBlitzes 2024

ABOL BioBlitz, Citizen Science, Highlights, Veranstaltungen

Thanks to the Biodiversity Fund projects ABOL-RefDat and GeMonA+, our ABOL team at the Natural History Museum Vienna grew at the beginning of 2024 and the first BioBlitzes were a good opportunity to support data collection and networking in the biodiversity community.

This year’s Day of Biodiversity of the Biosphere Reserve Wienerwald took place in Vienna-Liesing, at the same time part of the ABOL team traveled to the ÖEG insect camp in the Karwendel Nature Reserve.

On the occasion of the City Nature Challenge, an excursion to the Vienna-Liesing study area around Himmelswiese started at the end of April. Some species were already recorded there and on the Days of Biodiversity on June 8th and 9th this list of species was further expanded. In ideal weather conditions, which only made catching insects difficult due to occasional gusts of wind, we went along forest paths and extensive meadows to different types of water. The bright expert T-shirts meant that we kept meeting interested visitors or experts from other species groups, which led to nice conversations.

At the Biodiversity Festival, we shared an information stand with the Mycological Society, which was packed thanks to the favorable mushroom weather beforehand!

The Day of Biodiversity came to a cozy end at a nearby wine tavern, to which all experts were invited. A big thank you to the organizing team, as always it was a great experience that ran smoothly!

Impressions (photos: M. Sonnleitner)

16. July, 2024/by Michaela Sonnleitner

Our new project: ABOL-RefDat

Highlights, Projects

ABOL reference data (ABOL-RefDat) – call for project collaboration!

We are very pleased that the ABOL-RefDat project, funded by the Biodiversity Fund, started at the beginning of the year!

Our goal? The creation of at least 5,000 DNA barcodes for at least 1,500 Austrian species of animals, plants and fungi, as well as closing data gaps in Austria! A particular focus is on pollinators, soil and aquatic organisms.

As part of the RefDat project, we can award contracts for taxonomic work for the first time and are still looking for project partners!
Using checklists, we can perform GAP analyses to specifically identify taxon-specific data gaps, i.e. identify species for which no or only a few DNA barcodes of species from Austria are available in the BOLD (Barcode of Life Data System). This way we can work together to complete the reference data! As part of RefDat, collection material (that is not too old) can be used, as well as specifically newly collected material, for which collection permits must be available. The DNA barcodes generated in the project will be fed into the international database BOLD and will be available open access for numerous applications.

Requirements for cooperation:

suitable, morphologically well-determined material
submission of collection permits for new collected specimens (mandatory!)
deposition of reference organisms in scientific collections
start as soon as possible, sample processing should be completed by the beginning of 2025

Due to the relatively short project duration until October 2025, we are trying to find partners quickly.
If you are interested, please contact us as quickly as possible (abol@nhm-wien.ac.at)!

This project is funded by the Biodiversity Fund of the Federal Ministry for Climate Action, Environment, Energy, Mobility, Innovation and Technology of Austria.

23. January, 2024/by Michaela Sonnleitner

DNA barcoding workshop at UNZA - group photo

M. Sonnleitner

DNA barcoding workshop at the University of Zambia 2.0

Highlights, Meetings, Projects, Veranstaltungen, Workshops

Due to last year’s great success, the 2^nd DNA barcoding workshop took place in September at the University of Zambia (UNZA) in the capital Lusaka.
As part of the OeAD project “Inventorying biodiversity in the tropics: Establishing a DNA-barcoding pipeline for characterizing and monitoring Zambia’s biodiversity” led by the University of Graz (Stephan Koblmüller and Lukas Zangl) and participation by ABOL and Prof. Cyprian Katongo (UNZA ) students and staff of UNZA were trained in all the necessary steps to create DNA barcodes. The workshop began with a series of lectures on various aspects of DNA barcoding as a standard approach to recording biodiversity, its possible applications in monitoring, the challenges of a national biodiversity initiatives and opportunities for students and doctoral candidates to conduct research in Austria. The remaining workshop days covered the entire process of creating DNA barcode references – from sampling to data analysis. The DNA barcodes generated from fish and dragonflies are intended to serve as a crystallization point around which a national initiative in Zambia can be formed. Using the dragonflies, the participants were also introduced to the preparation and identification of insects. Laboratory work with DNA extraction and PCR, as well as the processing and evaluation of sequence data, were particularly popular. Overall, the workshop was once again a great success with around 30 participants.

DNA barcoding workshop at UNZA - data analysis

DNA barcoding workshop at UNZA - lab work

DNA barcoding workshop at UNZA - Prof. Katongo (UnNZA), Dr. Koblmüller (Univ. Graz)

Preparation and determination of dragonflies for DNA barcoding

DNA barcoding workshop at UNZA - collecting dragonflies & damselflies at the Goma lakes, UNZA

Collecting dragonflies at the university lake, UNZA

DNA barcoding workshop at UNZA - tissue sample

Collecting dragonflies at the Kafue river

DNA barcoding workshop at UNZA - collecting dragonflies at the Kafue river

DNA barcoding workshop at UNZA - lecture series: Dr. Zangl

DNA barcoding workshop at UNZA - lecture series: Dr. Koblmüller

DNA barcoding workshop at UNZA - collecting trip to the Kafue river

DNA barcoding workshop at UNZA - lecture series: Dr. Semani

Fish from the Kafue river for DNA barcoding

DNA barcoding workshop at UNZA - Prof. Katongo (UNZA)

19. October, 2023/by Michaela Sonnleitner

ABOL BioBlitz in the National park Hohe Tauern

ABOL BioBlitz, Highlights, Meetings, Veranstaltungen

This year’s ABOL BioBlitz series was completed with the Day of Biodiversity in the Hohe Tauern National Park on the Deferegger sunny side in East Tyrol. As in previous years, the weather this weekend was rather bad. At least this year we had some sunny windows in which flying insects, especially bumblebees, hoverflies and butterflies could be observed. The first evening was already dedicated to the light traps – nocturnal insects were attracted and mapped with light traps on the banks of the Schwarzach river and in the alpine area at Speikboden. Saturday was devoted entirely to searching for species in the study areas above St. Veit and St. Jakob, up to high alpine locations. In the evening, nets for catching bats were set up around the House of Water of the National Park, which served as a base, with which, together with echolocation, 5 species of bats could be detected. At the final event on Sunday, the first results and provisional species numbers were presented. Thanks to the voluntary and unpaid work of around 70 experts, the data situation in this area, which had been rather sparse until then, was significantly improved. We hope to get some more samples for the creation of DNA barcodes and to successfully complete the ABOL-BioBlitz collecting season.
We would like to thank the national park team for the perfect organization of accommodation, food and transport!

Impressions from the study area:

TdA NP Hohe Tauern, Defereggen valley. Photo: M. Sonnleitner

TdA NP Hohe Tauern, Defereggen valley. Light trap. Photo: M. Sonnleitner

TdA NP Hohe Tauern, Defereggen valley. N. Szucsich at the closing event. Photo: M. Sonnleitner

TdA NP Hohe Tauern, Defereggen valley. Welcome. Photo: M. Sonnleitner

TdA NP Hohe Tauern, Defereggen valley. Myricaria germanica on the Schwarzach river. Photo: M. Sonnleitner

TdA NP Hohe Tauern, Defereggen valley. Species rich meadow. Photo: M. Sonnleitner

TdA NP Hohe Tauern, Defereggental. Photo: M. Sonnleitner

TdA NP Hohe Tauern, Defereggen valley. Insects on Apiaceae inflorescence. Photo: M. Sonnleitner

2. August, 2023/by Michaela Sonnleitner

TdA Nockberge - Bärengrube. Photo: O. Macek

ABOL-BioBlitzes in Tyrol and the Carinthian Nockberge Mountains

ABOL BioBlitz, Highlights, Meetings

On July 7 and 8, the ABOL team was present at two simultaneous events: the Day of Biodiversity in Tyrol and the GEO-Day of Nature in Carinthia.

At the Day of Biodiversity in Tyrol, in Leutasch, four very different and exciting study areas were examined for their biodiversity. In a moor area with orchid-rich fens and an intact mountain pine high moor, night was turned into day with numerous light traps – at least for the nocturnal insects and their researchers. In addition, many fireflies, which are rarely observed in such high numbers today, lit our way. The numerous butterfly experts were also present high on the mountain in the direction of Arnkopf, at a species-rich meadow and an interesting gravel area. The beautiful weather on Saturday contributed to rich observations of various animal groups. The final event on Saturday afternoon was well attended and very informative and interesting with a very well-prepared photo presentation which was put together in a very short time. Experts from various fields contributed briefly, from the geology of the study area, a short introduction to iNaturalist to a poem about a moth (Baptria tibiale).

First results were presented, there was a lot to find on the four survey plots: 11 orchid species, about 200 different butterflies, including the rare dusky large blue (Phengaris nausithous), more than 50 bird species, including black grouse, golden eagle and tawny owl, some recordings on the bat-detector and sundew and moorland hawker in the moorland. As part of the post-processing, some more exciting species will surely be added to the species list. Also, the culinary art and the interaction among the experts wasn’t missed out, before we started the long journey home, which was definitively worth it! Many thanks to the organizing team!

The other part of the ABOL team took part in the GEO Day of Nature in the Nockberge. With the ‘Bärengrube’ (translated ‘bear-cave’) near Innerkrems we went this year to north-exposed sites of the biosphere reserve, with a diverse geology and soils, from dolomite to mica schist and numerous boggy areas. Not in every organism group the call for ‘bear’ associations could be served as elegantly as the botanical Lycopodium, a pun that loses its punch line in English. Not even the butterfly experts were successful due to the lack of Arctiinae sightings. The familiar atmosphere and the perfect weather led to the fact that nevertheless nobody was grieved. Moreover, the sightings of all three grouse species consoled even the melancholic about the absence of species like Contarinia baeri.

Our thanks go again to the organizers of E.C.O., the biosphere reserve team and the Ökoteam for the perfect organization and for opening the wide field of bear puns.

Impressions from Leutasch in Tyrol:

Impressions from the Nockberge:

20. July, 2023/by Michaela Sonnleitner

ABOL BioBlitz at the ÖEG insect camp 2023

ABOL BioBlitz, Highlights, Meetings

This year ABOL participated for the second time in the ÖEG insect camp with a BioBlitz. This year the four-day camp took place in the picturesque scenery of the Salzburger Kalkhochalpen of the Naturepark Weißbach and led us to species-rich mountain meadows, forest edges, swamp meadows up to alpine lawns.

Before starting into the field, the variety of different fields of expertise became apparent in the form of the stored equipment: Leaf vacuums for bugs, cicadas and various hymenoptera, beetle traps, luminous traps for moths and other nocturnal animals, hoverfly-, wild bee-, butterfly- & dragonfly-nets, sieves for millipedes, mosquito traps and much more. All framed by identification literature, binoculars, taxidermy utensils and a wide variety of collecting tubes.

In a short ABOL-presentation, the relevance of an Austria-wide DNA reference database and DNA-based identification approaches were presented. Interested participants could pick up tubes prepared by the ABOL team to barcode their collected animals. A Malaise trap for a metabarcoding approach was also set up again.

The days were very suitable for professional exchange and a common barbecue with an enjoyable evening in the naturepark rounded off the program wonderfully.

It can be said that the goal of the ÖEG, the promotion, documentation and representation of scientific entomology in Austria, was fully achieved during the four days. A big thank-you to the youth referent of the ÖEG Elisabeth Huber and the team of the Naturepark Weißbach, the schedule was flawless! Thank you very much for having us!

Impressions:

ÖEG insect camp 2023. Rock face on the way to Asenmoos. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Hochgrub, Leoganger Steinberge. Entomologist working in an alpine habitat. Photo: M. Sonnleitner

ÖEG insect camp 2023. Rock face on the way to Asenmoos. Photo: V. Kargl

ÖEG Insect camp 2023. Falleck. Species rich meadow. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Hochgrub, Leoganger Steinberge. Entomologists working in an alpine habitat. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Niedergrub, Leoganger Steinberge. Species rich pasture. Foto: M. Sonnleitner

ÖEG Insect camp 2023. Barbecue at the Weisbach creek. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Niedergrub. Leoganger Steinberge. Pasture with Primula farinosa. Photo: M. Sonnleitner

Hochgrub, Leooganger Steinberge. Alpine habitat. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Hochgrub, Leoganger Steinberge. Entomologist. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Hochgrub, Leoganger Steinberge. Clematis alpina. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Hochgrub, Leoganger Steinberge. Photo: M. Sonnleitner

Hochgrub, Leooganger Steinberge. Alpines Untersuchungsgebiet. Foto: M. Sonnleitner

ÖEG insect camp 2023. Grasenberg near Weißbach. Steep, rocky windbreak area. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Hochgrub, Leoganger Steinberge. Entomologists. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Falleck. Steep meadow. Photo: M. Sonnleitner

ÖEG Insect camp 2023. Hochgrub, Leoganger Steinberge. Alpine habitats. Photo: M. Sonnleitner

ÖEG Insect Camp 2023. Barbecue at Weißbach, Hintertal. Photo: M. Sonnleitner

ÖEG insect camp 2023. Collecting soil animals. Photo: V. Kargl

ÖEG insect camp 2023. Enallagma cyathigerum. Photo: V. Kargl

ÖEG insect camp 2023. Rainbow in Lofer. Photo: M. Sonnleitner

ÖEG insect camp 2023. Rhyssa persuasoria, sabre wasp. Photo: V. Kargl

ÖEG insect camp 2023. Malaise trap for metabarcoding. Falleck – steep meadow. Photo: M. Sonnleitner

3. July, 2023/by Michaela Sonnleitner

M. Sonnleitner

The first two ABOL BioBlitzes of 2023!

ABOL BioBlitz, Highlights, Meetings, Veranstaltungen

The first two Days of Biodiversity took this year place in Vienna (allotment Villaweg) and Lower Austria (Klosterneuburg, Biosphere Reserve Wienerwald).

In Klosterneuburg, we were exploring species-rich meadows, natural Wienerwald brooks, or the biosphere reserve core zone “Dombachgraben” in fantastic early summer weather. In Vienna, too, the weather left nothing to be desired – only the floodlights of the nearby soccer field competed in the evening with the insect light traps and bat catching, including a night tour. Nevertheless, a Viennese peacock moth (Saturnia pyri) could be observed at a light trap, a highlight for everyone!

While in Vienna all sites in and around the Villaweg allotment were easily accessible on foot, the area in Klosterneuburg, on the northern edge of the Biosphere Reserve Wienerwald, extended over several square kilometers and a shuttle service was provided. The species search started on Friday morning and ended on Saturday noon in a festival of biodiversity with information booths and expert excursions!

Even though DNA barcoding may appear to be a more complex topic at first glance, our ABOL information booth was well visited and we could inform visitors about the different activities of ABOL as well as win experts for the ABOL BioBlitz! At this point we would like to thank the organizers for the great organization!

Impressions:

Day of Biodiversity in Klosterneuburg - collecting species

7. June, 2023/by Michaela Sonnleitner

9th ABOL meeting 2022 – a retrospect

Highlights, Meetings

After 2 years of virtual conferences, we were very happy to be able to hold the ABOL annual conference physically again this year. On November 18^th the time had come. The 9^th ABOL conference took place in the Haus für Natur, Museum Niederösterreich, after the 5^th Austrian Biodiversity Forum. As usual, the lecture program was exciting and very broad in topics. The inspiring keynote lecture by Natasha de Vere from the University of Copenhagen on pollen metabarcoding was one of the morning highlights, followed by lectures on different applications of metabarcoding and eDNA barcoding. We were also very pleased to welcome Cyprian Katongo from the University of Zambia to the ABOL meeting and hear about planned DNA barcoding activities in Zambia. The focal points in the afternoon were the detection of disease vectors using DNA barcoding, but also various groups of organisms such as molluscs, butterflies, gentians and the impressive dung fungi. We would like to thank all the speakers for their exciting contributions and the participants for the lively discussions. Unfortunately, with around 55 participants, the conference was less well attended than the conferences before the pandemic.
Special thanks also go to the Museum Niederösterreich for the good cooperation and our sponsors Microsynth, Eppendorf and MDPI Diversity.